HTS of MARV passaged isolate - SRA

SRX21227671: HTS of MARV passaged isolate
1 ILLUMINA (Illumina MiSeq) run: 52,886 spots, 11.3M bases, 3.8Mb downloads

Design: Viral RNA was extracted using a Qiagen's QIAmp Viral RNA Mini Kit or RNeasy Mini Kit. The purified RNA served as template to generate cDNA using the SuperScript IV First-Strand Synthesis System (Invitrogen), and was subsequently turned into double-stranded DNA with the NEBNEXT mRNA Second Strand Synthesis Module (New England Biolabs). Target enrichment using in-solution hybridization capture (myBaits Hybridization Capture for Targeted NGS protocol ) was used for deep-sequencing.

Submitted by: Robert Koch institute

Study: Filovirus sequencing

PRJNA1001398 • SRP453002 • All experiments • All runs

show Abstracthide Abstract

Sequences of in vitro and in vivo passaging of filoviruses. The goal is to evaluate virus adaptation to different hosts.

Sample:

SAMN36810537 • SRS18483392 • All experiments • All runs

Organism: Orthomarburgvirus marburgense

Library:

Name: DM6

Instrument: Illumina MiSeq

Strategy: WGS